LOCUS       pLV-[hsa-miR-548d-2]        9293 bp    DNA     circular     27-JUN-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7214..8074
                     /label=Amp(R)
     promoter        8275..8815
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5819..6053
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6628..7083)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    6125..6255
                     /label=SV40 pA
     misc_feature    6256..6469
                     /label=SV40 ori
     misc_feature    5140..5736
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3758
                     /label=intron
     CDS             3805..5130
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3548
                     /label=hsa-mir-548d-2 precursor
BASE COUNT     2253 a      2372 c      2499 g      2169 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatcca gtacgatatt 
     3121 aacattgggg gaaggtggat gaagactacg gggggttctc tggatttgca actcttctgt 
     3181 aaatctaaag ttaactttag ggggtatatg atgtcaacct gatttagcta aagttaactt 
     3241 tagatttaca gaataattgc aaatccagag agagggaaga tttaggttgg tgcaaaagta 
     3301 attgtggttt ttgccattga aagtaatggc aaaaaccaca gtttcttttg caccaaccta 
     3361 ataaaataaa aagtttaaaa ggaaaaaaat ctctcaaatg aagacgtagg tccacacaag 
     3421 gacttgtatg tgaatgttca ttgcaacttt actcaaaatc atccaaaact ggaagtaacc 
     3481 caaatgtcca tcaactggta aatgaataaa cagactgtgg tatatccatt caggggaatg 
     3541 ctctatgcgc tagctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt 
     3601 atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca gcttggcact 
     3661 tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc 
     3721 ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgaaaa ctacccctct 
     3781 agagtcgagc taccggtcgc caccatggtg agcaagggcg aggaggataa catggccatc 
     3841 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc 
     3901 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag 
     3961 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac 
     4021 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc 
     4081 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg 
     4141 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc 
     4201 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc 
     4261 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg 
     4321 aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg 
     4381 cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac 
     4441 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac 
     4501 gagctgtaca aggacccacc ggtcgccacc atgaccgagt acaagcccac ggtgcgcctc 
     4561 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac 
     4621 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa 
     4681 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc 
     4741 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag 
     4801 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa 
     4861 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc 
     4921 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg 
     4981 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc 
     5041 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagt gcccgaagga ccgcgcgacc 
     5101 tggtgcatga cccgcaagcc cggtgcctga gcggccgcgt cgacaatcaa cctctggatt 
     5161 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg 
     5221 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct 
     5281 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc 
     5341 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca 
     5401 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac 
     5461 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt 
     5521 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct 
     5581 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc 
     5641 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga 
     5701 cgagtcggat ctccctttgg gccgcctccc cgcctggaat tcgagctcgg tacctttaag 
     5761 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact 
     5821 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct 
     5881 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 
     5941 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 
     6001 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag 
     6061 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga 
     6121 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 
     6181 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 
     6241 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact 
     6301 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 
     6361 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 
     6421 ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg 
     6481 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac 
     6541 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac 
     6601 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg 
     6661 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt 
     6721 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc 
     6781 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg 
     6841 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga 
     6901 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc 
     6961 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa 
     7021 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa 
     7081 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat 
     7141 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg 
     7201 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc 
     7261 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga 
     7321 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga 
     7381 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg 
     7441 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc 
     7501 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac 
     7561 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact 
     7621 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca 
     7681 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg 
     7741 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact 
     7801 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg 
     7861 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg 
     7921 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat 
     7981 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc 
     8041 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat 
     8101 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt 
     8161 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc 
     8221 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt 
     8281 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac 
     8341 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt 
     8401 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct 
     8461 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga 
     8521 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac 
     8581 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg 
     8641 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt 
     8701 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc 
     8761 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg 
     8821 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc 
     8881 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc 
     8941 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag 
     9001 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca 
     9061 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat 
     9121 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg 
     9181 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga 
     9241 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgc 
//
